Genetic Guidelines for Conservation Collections

Katie Heineman's picture
Given the reproductive difficulties that differences in plant ploidy may cause in the movement or mixture of plant populations, our group is considering incoporating analysis of plant ploidy into a grant evaluating
posted by Katie Heineman on Tuesday, August 27, 2019 - 11:20am
We are testing DNA extraction protocols for Dudleya brevifolia and are able to obtain a small amount of DNA in some trials, though the same conditions do not always work consistently across multiple samples.  Thus far we have tried combinations of various protocols (QIAGEN kits, a CTAB protocol), prewash steps, tissue amounts, tissue types (whole leaf tissue, epidermal tissue, parenchymal tissue).  Another plant tested alongside Dudleya extracted well, so there shouldn't be a problem with the reagents. We're going to try flower buds next.  Any additional suggestions are much apprec
posted by Stephanie Steele on Thursday, April 25, 2019 - 2:00pm