seed storage

Information on Rotala ramosior seed and storage

We had a potential project with salvaging Rotala ramosior (annual) pop up and we are hoping to quickly find information on this plant so we can advise/store properly. Has anyone worked with this species (or genus) before? A wetland annual plant, we are hoping to know about when best to collect, how best to prep and store, both long- and short-term, and how to best maximize germination. Any experiences working with this species would be most welcome.

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Foil pouch prices from Flex-Pak

Hi all, 

In following Heather's post from yesterday, I wanted to share some information and prices that I received from Flex-Pak. Below is the email from them:

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Seana Walsh, Dustin Wolkis, and Ken Wood, National Tropical Botanical Garden

Phyllostegia electra (Lamiaceae) is endemic to the mesic and wet forests of Kaua'i. It is listed as Critically Endangered on the IUCN Red List of Threatened Species and is a focal species for achieving conservation objectives outlined in the Hawai'i Strategy for Plant Conservation. With less than 50 known wild individuals among 15 subpopulations, P. electra is also a focal species of the University of Hawai'i's Plant Extinction Prevention Program. It is not, however, protected by the Endangered Species Act.

A grant from the Mohamed bin Zayed Species Conservation Fund is supporting NTBG staff to: 1) make conservation collections from wild populations, 2) conduct a genetic diversity study in collaboration with Chicago Botanic Garden (CBG), 3) outplant into protected and managed habitat, and 4) investigate optimal seed storage methods.

Eighteen remote field work trips have been undertaken since March 2017 to secure conservation collections and obtain leaf material for the genetic diversity study. Genetic marker (microsatellites) development was recently completed and silica-dried leaf material sent to CBG for DNA extraction. Since June 2017, 215 individuals have been outplanted into Kalalau Exclosure and NTBG Gardens and Preserves. Preliminary results of our investigation into optimal seed storage indicate that seeds do not tolerate exposure to liquid nitrogen without prior desiccation. We also found that germination was significantly higher in the 42% eRH frozen treatment compared to the 30% eRH frozen treatment. This work is directly contributing to the conservation of this rare taxon and we are using this multi-faceted project model in our approach to conserving other rare plant taxa as well.

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Date Recorded: 
Friday, May 3, 2019

Kim Taylor, Botanical Research Institute of Texas / Fort Worth Botanic Garden

The Botanical Research Institute of Texas (BRIT) is in the process of constructing a seed conservation laboratory and seed bank. While BRIT has been active in conservation for decades and has partnered with regional seed banks to conserve rare species, BRIT has never had a dedicated seed laboratory on site. The goal of the BRIT Seed Conservation Laboratory will be to collect and maintain ex situ collections for all rare plant taxa occurring in Texas. In order to equip the laboratory with the appropriate equipment and begin a strategic plan to collect all rare Texas taxa, we first need to understand the seed storage behavior of the taxa of concern. An assessment of the seed storage behavior of 449 vascular plant taxa listed by Texas Parks and Wildlife as Species of Greatest Conservation Need will be discussed, including how the results impacted design of the BRIT seed bank.

Contributing Author(s): 
Date Recorded: 
Thursday, May 2, 2019