Gayle Volk, USDA ARS National Laboratory for Genetic Resources Preservation, United States

The USDA-ARS National Plant Germplasm System has over 30,000 clonally maintained accessions within its field, screenhouse, greenhouse, and tissue culture collections. These fruit, nut, tuber, and bulb crop collections are usually not duplicated at secondary locations and are vulnerable to bioticabiotic, and climatic threats. Only about 15% of the clonally maintained accessions are currently secured in long-term storage at the National Laboratory for Genetic Resources Preservation (NLGRP) in Fort Collins, Colorado. The labor required to cryopreserve the clonal collections at NLGRP exceeds that which is available, even when reliable, robust cryopreservation methods are available. We have sought to prioritize collection materials for cryopreservation and to identify methods that improve the efficiency of the shoot-tip cryopreservation procedure. In particular, we have used field-, screenhouse-, and growth-chamber harvested plant tissue as source material for shoot tip cryopreservation, rather than relying on in vitro grown cultures. This strategy has been particularly effective for garlic, citrus, and grape cryopreservation efforts. In addition, incorporation of antioxidants and shoot tip micrografting methods have made cryopreservation protocols widely applicable to diverse genetic resources for each crop.

Contributing Author(s): 
Date Recorded: 
Tuesday, July 23, 2019

Dr. Jennifer Ramp Neale, Director of Research and Conservation, Denver Botanic Garden 

The primary objective of research at Denver Botanic Gardens is the conservation, preservation, and documentation of native Colorado flora by serving as an active center of biodiversity research for the Southern Rocky Mountain region. In order to fill a gap in botanical expertise in the region, the Gardens launched a conservation genetic program in 2009. Through an integrated approach we have assessed population genetic diversity levels and patterns in several endangered plant species. We are now expanding our skill set through staffing, collaboration, and student mentorship. Staying on top of current methodology while providing results to funding agencies in a timely manner has its challenges that we are working to address in creative ways.

Contributing Author(s): 
Date Recorded: 
Tuesday, September 10, 2019

Assistant Professor in Plant Evolutionary Biology - University of Colorado Denver, College of Liberal Arts and Sciences

Hi Everyone -

 The University of Colorado Denver is hiring a plant evolutionary biologist withiin the department of Integrative Biology. Several staff at Denver Botanic Gardens hold adjoint faculty status with the department and mentor graduate students through the University. This position will work closely with staff at Denver Botanic Gardens. Please share this posting with anyone in your network who may be interested.

Review of applications will begin 11/30/2019

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